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TG2 activity is demonstrated with 5-biotin pentylamine labeling in a cultured human epithelial organoid. The TG2 inhibitor ZED1227 (0.02 mg/mL) caused a reduction of 5-BP staining intensity in the apical epithelial surface compared to the DMSO control (arrows). Panel (A) (negative control); panel (B) (DMSO control; no TG2 inhibitor added); panels (C,D) (with ZED1227 0.002 mg/mL and 0.02 mg/mL added, respectively).
