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. 2023 Jul 3;24(13):11035. doi: 10.3390/ijms241311035

Figure 2.

Figure 2

Amino acid sequences of VH-Linker-VL of CCK-B-specific mouse scFvs using Clustal Omega. FRs and CDRs are determined by the IMGT information system. Diversity was found predominantly in the CDR regions. A typical 20-amino-acid linker [(G4S)4] joins the VH and VL chains. Alignments were color coded according to residue property groups. AVFPMILW—red, DE—blue, RK—magenta, STYHCNGQ—green, others—grey. “*” (asterisk) indicates positions which have a single, fully conserved residue; “:” (colon) indicates conservation between groups of strongly similar properties; “.” (period) indicates conservation between groups of weakly similar properties.