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. 2023 Jul 3;24(13):11035. doi: 10.3390/ijms241311035

Figure 15.

Figure 15

Effect of scFv 77-2 on DRG neuron excitability. (A) Representative current clamp recording of a DRG neuron showing response to 10 pA stepwise current injection from rheobase. (B) Number of action potentials (APs) vs stepwise current injection (above rheobase) of DRG neurons following treatment with CCKBR scFv 77-2 (10 ug/mL) or CCKBR antagonist LY225910 (100 nM) compared to vehicle controls (water for scFv and DMSO for LY225910. Only multi-firing neurons could be used for this analysis n = 6 Vehicle (water), n = 6 scFv 77-2, n = 8 Vehicle (DMSO) and n = 6 LY225910. Inset: Comparison of data from plot. (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. One-way ANOVA with post hoc Tukey’s multiple comparisons test). (C) No statistically significant differences in rheobase, resting membrane potential or input resistance was observed between conditions. Total n = 8 Vehicle (water), n = 7 scFv 77-2, n = 8 Vehicle (DMSO) and n = 9 LY225910.