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. 2023 Jun 26;24(13):10646. doi: 10.3390/ijms241310646

Table 1.

Agrobacterium-mediated transformation of monocots and dicots.

Species Explants Genotype Target Gene Agrobacterium Strain Vector Selectable Marker Efficiency (%) Reference
Monocots
Wheat Immature embryos Bobwhite SH98 26 sGfp AGL1 pGH215 Hpt Up to 15% [26]
Immature embryos Fielder gus AGL1 pGoldenGreenGate Hpt Up to 25% [25]
Immature embryos CB037, Fielder, Jimai 22
Kenong 199, Shi 4185
gus C58C1 pRK2013 Bar 2.8–53% [28]
Mature and immature embryos DBW 88, DBW 90, DBW 93, DPW 621-50, HD 3086
and WH 1105
gus EHA105 pCAMBIA3301 Bar 9.8–14.9% [27]
Rice Immature embryos a broad range of
species
gfp LAB4404 and EHA105 pPUG1-1 Hpt - [29]
Embryogenic calli Sambha mahsuri AmSOD1 LAB4404 and EHA105 pSFSOD1 Hpt - [30]
Maize Immature embryos ND101 and ND88 DsRed EHA105 Bar Up to 17.6% [31]
Immature embryos HC69 and PH2RT YFP LBA4404THY- pVIR PAT/PMI - [24]
Barley Immature embryos Scarlett and Golden Promise HvCKX2 AGL1 pMCG161 Bar 3.47% [32]
Immature embryos Golden Promise gus AGL1 pBRACT Hpt 25% [33]
Dicots
Eucalyptus Leaves E. urophylla × E. grandis clone DH32-
29
gus GV3101, LBA4404, EHA105, and
AGL1
pBI121 Npt II 1.9% [34]
Poplar Leaves Populus Alba×Populusglandulosa Uyeki gus GV3101 35S:GUS vector - - [35]
Callus 84K gus GV3101 pCAM
BIA1301
Hygromycin B greater than 50% [36]
Codonopsis pilosula Stems (Franch.) Nannf. gus GV3101 pCAMBIA1381 Hpt 91.07% [37]
Tea Callus Camellia
sinensis
gus EHA105 PS1aG-3 - 3.6% [38]
Liriodendron hybrid Callus 52053 gus EHA105 pBI121 Geneticin 60.7% [39]
Pigeon pea Cotyledons ICPL
85063
gus/gfp LBA4404 pCAMBIA1301 Hpt 83% [40]
Soybean Cotyledonary node Jack GsWRKY20 EHA101 myc-pBA Bar - [41]
Ailanthus altissima (Mill) Swingle Shoots - gfp K599 pCAMBIA1300 - - [21]
Cotton Shoot apical meristem Gossypium hirsutum, Gossypium barbadense and Gossypium
arboreum
GFP and RUBY - pCAMBIA 2300 AADA Up to 8.01% [42]