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. 2023 Jun 22;24(13):10474. doi: 10.3390/ijms241310474

Figure 1.

Figure 1

LRRK2 KO decreases the expression of LRRK2 protein. (A,B) Representative flow cy-tometry contour plots show total LRRK2 protein in LRRK2 WT and LRRK2 KO cells. (C) Percent-age of total LRRK2 protein in LRRK2 WT and LRRK2 KO cells. The data are presented as the mean ± SD of three independent experiments (n = 3). Student’s t-test: Statistically significant differences when *** p < 0.001. Additionally, HEK-293 LRRK2 WT cells and KO cells were stained with Hoechst 33342 (D,E), primary antibodies against total-LRRK2 (F,G), and merged (H,I). Positive blue fluorescence reflects nuclei, and positive red fluorescence reflects total-LRRK2. (J) Quantification of the mean fluorescence intensity (MFI) in WT and KO cells. The figures represent one out of three independent experiments. One-way ANOVA, post hoc test Bonferroni. The data are expressed as the mean ± SD; *** p < 0.001. Image magnification, 200×.