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. 2023 Jul 1;24(13):10983. doi: 10.3390/ijms241310983

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative mRNA and protein expressions of acetylated enzymes (IDH2, LDHC, MDH2 and SIRT5) in fresh and post-thawed boar sperm. (A) WB analysis indicates protein expression between fresh and frozen (post-thawed) sperms; (B) mRNA expression of IDH2, LDHC, MDH2 and SIRT5 between fresh and post-thawed sperms; (C) relative protein expressions of IDH2, LDHC, MDH2 and SIRT5 between fresh and post-thawed sperms; (D) WB analysis of SIRT5 in fresh, frozen, siSIRT5 and NC groups; (E) relative gene expression of SIRT5 in fresh, siSIRT5 and NC groups; (F) relative protein expression of SIRT5 in fresh, frozen, siSIRT5 and NC groups. All the data were subjected to statistical analysis and considered significant at (* p < 0.05) and highly significant at (** p < 0.01 and *** p < 0.001). Isocitrate dehydrogenase (IDH2); malate dehydrogenase (MDH2); sirtuin5 (SIRT5); lactate dehydrogenase (LDHC).