Table 2.

Assessment of epigenome-related effects after TTC.

Reference	Type/Species (Cryopreservation Technique)	Analysis (Technique)	Main Outcome
Oblette et al. (2019) [83]	Murine in vitro culture of TT (solid surface vitrification including 2.1 mol/L DMSO and 2.7 mol/L EG or slow freezing including 1.5 mol/L DMSO)	Testicular tissue after IVC (qPCR and immunofluorescent assay)	Normal expression of epigenetic modification enzymes and gDNA methylation, but differences in histone modification
Oblette et al. (2021) [82]	Murine in vitro culture of TT (slow freezing including 1.5M DMSO)	Pre-implantation embryo (immunofluorescent assay)	Normal post-translational histone modifications and altered gDNA methylation