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. 2023 Jun 30;24(13):10891. doi: 10.3390/ijms241310891

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Glycosylation pattern of CD19-AD2 expressed in CHO-K1 cells cultivated at 37 °C vs. 32 °C. The five N-glycosylation sites at position N67, N106, N119, N162, and N246 are characterized. (A) Color-coded bar graph of the site-specific glycosylation as relative proportions of found glycoforms with a table legend. (B) Representative structures of sialylated N-glycans found and their percentual representation are displayed extra in table format (threshold of prevalence > 4.5%).

Glycosylation pattern of CD19-AD2 expressed in CHO-K1 cells cultivated at 37 °C vs. 32 °C. The five N-glycosylation sites at position N67, N106, N119, N162, and N246 are characterized. (A) Color-coded bar graph of the site-specific glycosylation as relative proportions of found glycoforms with a table legend. (B) Representative structures of sialylated N-glycans found and their percentual representation are displayed extra in table format (threshold of prevalence > 4.5%).