Table 1.

Kinetic binding constants of various immobilized anti-CD19 antibody ligands binding to the CD19-AD2 analyte in solution. Values are obtained after the global fitting of the binding signals obtained by BLI measurements. Signals detected above the level of non-specific binding were listed.

Analyte in Solution	Immobilized Ligand ID	kon 105/Ms	koff [10−4/s]	KD (nM)
CD19-AD2	FMC63	2.06	6.57	3.19
	HIB19	0.44	16.99	38.29
	3B10	0.11	2.16	19.12
	4G7	no spec. binding