Table 2.
Determination of the AAV subpopulations of an AAV9 sample comprising ~33% empty, ~6% partially filled, ~52% full, and ~8% overfull particles. The sample was measured five times on three consecutive days (days 1–3). The obtained results were compared to AUC data. E, PF, F and OF stand for empty, partially filled, full and overfull, respectively.
| MP | AUC | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Average E/% | RSD/% | Average PF/% | RSD/% | Average F/% | RSD/% | Average OF/% | RSD/% | E/% | PF/% | F/% | OF/% | |
| Day 1 | 39.7 | 5.4 | 7.1 | 8.7 | 49.7 | 2.2 | 3.5 | 9.8 | 33.1 | 6.3 | 52.3 | 8.4 |
| Day 2 | 40.0 | 6.8 | 8.8 | 9.8 | 47.8 | 8.6 | 2.8 | 5.4 | ||||
| Day 3 | 40.4 | 3.8 | 8.3 | 1.8 | 48.1 | 3.1 | 2.3 | 8.7 | ||||