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. 2023 Jul 1;24(13):10999. doi: 10.3390/ijms241310999

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Steps of somatic embryogenesis and plant regeneration in banana. (a) Multiple meristems (also known as scalps) explants cultured for callus induction, (b) Male bud cultured for callus induction, (c) Friable embryogenic ideal callus, (d) Embryogenic cell suspension, (e) Embryogenic calli on embryo development medium, (f) Embryogenic calli on embryo maturation medium, (g) Embryos germinating in the dark, (h) Embryos germinating into plantlets and shoots turning green upon transferring them to light, (i) Fully developed plantlets with roots, (j) Complete plants transferred to pots in the greenhouse. Scale bar: 1 cm in (a,b,d–i), 10 cm in (j), and 5000 µm in (c).