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. 2023 Jun 22;24(13):10486. doi: 10.3390/ijms241310486

Figure 2.

Figure 2

Analysis of ClNAC100 transcriptional activity: (a) Cloning of ClNAC100 NAC domain and TR-region. (b) Validation of transcriptional autoactivation of ClNAC100: different truncations of ClNAC100 fused to the GAL4 DNA binding domain in pGBKT7 vector. Transactivation activity assay of ClNAC100 in yeast strain Y2H Gold on the SD/-Trp/-Leu/-Ade/X-α-gal medium.