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. 2023 Jun 27;24(13):10716. doi: 10.3390/ijms241310716

Figure 5.

Figure 5

Figure 5

Sophora japonica extracts before (KPF-BBR) and after bioconversion reactions (KPF-ABR) inhibit cell proliferation and seem to have an apoptotic effect on grade III glioma cell lines. (A) KPF-BBR and KPF-ABR treatments significantly inhibit the proliferation rate of NG-97 and U251 cells in a time-dependent way, compared with the untreated control cells. (B) NG-97 cells are incubated with 200, 400, and 600 µM of KPF-BBR, and 600, 800, and 1000 µM of KPF-ABR for 72 h. (C) U251 cells are incubated with 1600, 1800, and 2000 µM of KPF-BBR and KPF-ABR for 72 h. Genomic DNA is isolated and analyzed on a 1.5% agarose gel stained with ethidium bromide. M: DNA marker 100 base pairs; C: untreated control cells. (D) Representative results showing the percentage of NG-97 cells stained with AnnexinV-PE/7-AAD after TMZ (positive control) [4000 µM], KPF-ABR [600 µM], or KPR-BBR [800 µM] treatments for 72 h. The percentage of apoptotic cells is indicated. (E) Representative results showing the percentage of U-251 cells stained with AnnexinV-PE/7-AAD after TMZ (positive control) [4000 µM], KPF-ABR, or KPR-BBR [1800 µM] treatments for 72 h. The percentage of apoptotic cells is indicated. The data presented are the mean ± standard deviation of the experiments performed in triplicate. The T-test indicate p-values ≤ 0.05.