Table 1.
Changes in the relative abundances and putative identification of Saphora japonica compounds after enzymatic hydrolysis.
| Index | Feature | Putative ID | Formula | Adducts | Ionization Mode | Trend |
|---|---|---|---|---|---|---|
| 1 | 2.04_595.1563 m/z | Quercetin-3,7-O-α-L-dirhamnoside | C27H30O15 | M+H | + | DOWN |
| 2 | 2.16_741.2171 m/z | Kaempferol-3-Galactoside-6″-Rhamnoside-3‴-Rhamnoside a | C33H40O19 | M+H | + | DOWN |
| 3 | 2.35_595.1632 m/z | Kaempferol-3-O-glucoside-3″-rhamnoside b,c** | C27H30O15 | M+H | + | DOWN |
| 4 | 2.39_615.1052 m/z | Kaempferol-3-O-rhamnoside-7-O-rhamnoside b | C27H30O14 | M+K-2H | - | DOWN |
| 5 | 2.44_609.1464 m/z | Kaempferol 3-O-sophoroside d* | C27H30O16 | M-H | - | DOWN |
| 6 | 2.46_255.0672 m/z | Daidzein | C15H14O6 | M+H-2H2O | + | UP |
| 7 | 2.53_755.2084 m/z | Quercetin-3-O-α-L-rhamnopyranosyl(1-2)-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside | C33H40O20 | M-H | - | DOWN |
| 8 | 2.54_477.0638 m/z | Quercetin 3-glucuronide | C21H18O13 | M-H | - | DOWN |
| 9 | 2.55_473.1010 m/z | Flavanomarein | C21H22O11 | M+Na | + | UP |
| 10 | 2.75_299.0516 m/z | Isorhamnetin | C14H12O6 | M+Na | + | UP |
| 11 | 2.77_455.0965 m/z | Genistein 7-O-glucoside | C21H20O10 | M+Na | + | UP |
| 12 | 3.22_359.0732 m/z | 5-caffeoylshikimic acid | C16H16O8 | M+Na | + | UP |
| 13 | 3.27_431.0949 m/z | Kaempferol 3-O-galactoside a | C21H20O11 | M+H-H2O | + | DOWN |
| 14 | 3.30_370.0882 m/z | Gallocatechin | C16H17N3O4S | M+Na | + | UP |
| 15 | 3.36_391.0328 m/z | Chlorogenic acid | C21H20O10 | M-H | - | UP |
| 16 | 3.36_407.0710 m/z | Epicatechim galate | C22H18O10 | M+H-2H2O | + | UP |
| 17 | 3.37_433.1128 m/z | Kaempferol-3-O-rhamnoside a | C21H20O10 | M+H | + | DOWN |
| 18 | 3.44_431.0935 m/z | Kaempferol 3-glucoside a | C21H18O10 | M+H | + | UP |
| 19 | 3.70_270.0504 n | Apigenin | C15H10O5 | M+H-H2O | + | UP |
| 20 | 3.71_271.0948 m/z | Medicarpin | C16H14O4 | M+H | + | UP |
| 21 | 3.87_317.0649 m/z | Quercetin 4′-methyl ether (Tamarixetin) | C16H12O7 | M+H | + | UP |
| 22 | 4.50_609.1555 m/z | Rutin ** | C27H30O16 | M-H | - | DOWN |
| 23 | 4.17_495.1275 m/z | Catechin | C24H24O10 | M+Na | + | UP |
| 24 | 4.83_271.0577 m/z | Genistein | C15H10O5 | M+H | + | UP |
| 25 | 5.74_302.0400 n | Quercetin | C15H10O7 | M+H-H2O, | + | UP |
| 26 | 6.51_285.0409 m/z | Kaempferol | C15H10O6 | M-H | - | UP |
a also identified in the negative mode; b MSI level 3; c or Kaempferol-3-O-galactoside-7-O-rhamnoside; d p-value obtained after Pattern−Hunter statistical analysis. For all comparisons, p-value < 0.05 except for * p-value < 0.1 and ** p-value < 0.5.