Figure 1.

The administration of metformin in mice subjected to restraint stress rescued the up-regulation of circulating P-selectin^hi platelets. (A) Experimental outline illustrating the restraint-stress mouse model and platelet analysis. (B) Flow cytometry analysis was performed to determine the percentage of platelets expressing high surface levels of P-selectin (P-selectin^hi) in mice subjected to restraint stress, with or without metformin treatment at 0 and 9 h. The error bars represent the standard deviation. * p < 0.05 compared with the respective 0-h groups; # p < 0.05 compared with the respective stress + vehicle groups. The number of mice used in each group was 6 (three experiments with a total of 6 mice per group).