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. 2023 Jul 6;24(13):11153. doi: 10.3390/ijms241311153

Figure 5.

Figure 5

Western blot analysis for PI3K–AKT–MTOR and RAS/RAF/MAPK (MEK)/ERK pathways. (A) TPC1 cells were treated with basal conditions (5% FBS) or LDL (200 μg/mL ApoB) and harvested at 24 h, 48 h and 72 h. Last panel: representatives blots are shown. Stain-free (SF) gel was used as the loading control. Graphs show the densitometry of the Western blots relative to basal condition-treated cells in comparison to LDL-treated cells. (B) BCPAP cells were treated with basal conditions (5% FBS) or LDL (200 μg/mL ApoB) and harvested at 24 h, 48 h and 72 h. Last panel: representative blots are shown. Stain-free (SF) gel was used as the loading control. Graphs show densitometry of the Western blots relative to basal condition-treated cells in comparison to LDL-treated cells. Statistical analysis: two-way ANOVA test plus Sidak’s multiple comparisons test (* p < 0.01, ** p < 0.001). Data are expressed as mean ± SEM of a minimum of three independent experiments (N = 3).