Figure 6.

3-MA inhibited the activation of autophagy induced by resveratrol. Luteinized granulosa cells were treated with 20 μM resveratrol and 10 mM 3-MA and 200 μM H₂O₂ for 24 h. (A–F) The protein expression levels of Beclin1, Atg5, Lc3B II/I, Atg12, and Atg16L were detected using Western blotting and normalized to GAPDH (n = 3). (G–K) The mRNA expression levels of Beclin1, Atg5, Lc3B, Atg12, and Atg16L were detected using real-time PCR (n = 3). Compared with the control group, * p < 0.05, ** p < 0.01; compared with the H₂O₂ group, # p < 0.05, ## p < 0.01, ### p < 0.001; compared with the H₂O₂ + RSV group, & p < 0.05, && p < 0.01.