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. 2023 Jun 30;24(13):10958. doi: 10.3390/ijms241310958

Figure 2.

Figure 2

Quantification method for the autoantibody against β2GPI/HLA-DR complex. IgG binding to both β2GPI/HLA-DR complex-expressing cells (GFP-labeled) and HLA-DR-expressing cells (Ds-Red labeled) were analyzed using flow cytometry. Cells are gated on Ds-Red positive cells (A, a) or GFP positive cells (A, b). Specific IgG binding to the β2GPI/HLA-DR complex is calculated by subtracting the MFI of IgG binding to Ds-Red bright cells (B, c) from the MFI of IgG binding to GFP bright cells (C, d). A standard curve is generated from the specific IgG binding to β2GPI/HLA-DR complexes in sequentially diluted standard sera (D).