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. 2023 Jul 4;24(13):11077. doi: 10.3390/ijms241311077

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of the expression profiles by qRT-PCR. Nine differentially expressed genes, including five WOX genes (GH_A05G2053, GH_D05G1617, GH_A05G1589, GH_A02G2045, and GH_D03G0016), were selected to conduct qRT-PCR. The horizontal axis showed different timepoints of cotton somatic embryogenesis by T1 to T9, representing NEC (T1-T4), NEC/EC (transitional stage from NEC to EC) (T5), EC (T6), GE (T7), TE (T8), and CE (T9), respectively. The green columns represent the gene relative expression levels calculated by qRT-PCR; the dot-lines represent those calculated by TPM values.