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. 2023 Jul 5;24(13):11117. doi: 10.3390/ijms241311117

Figure 6.

Figure 6

ELF-EMS decreases microglia migration in organotypic hippocampal slice cultures (OHSC). OHSC from CX3CR1WT/eGFP mice were submitted to OGD and stimulated with ELF-EMS for four consecutive days. Ten images were taken during a 6 h period at day 1, 3 and 7 after OGD for quantifying microglia migration. (A) Quantification of microglial density in the CA1 area of the hippocampus in 10 time points during 6 h. Statistical analysis was done with Two-way ANOVA, Tukey (post-hoc) * p < 0.05, n = 4–6. (B) Quantification of microglia presence in the CA1 region of the hippocampus in the last time point during microglia migration monitoring. Statistical analysis was done with One-way Anova, Tukey (post-hoc) * p < 0.05, *** p < 0.01, **** p < 0.001, n = 4–6. (C) Representative images of microglia in green in the CA1 area of the hippocampus in the last time point during microglia migration monitoring at 1, 3 and 7 days. Scale bar = 10 µm.