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. 2023 Jul 6;24(13):11140. doi: 10.3390/ijms241311140

Figure 3.

Figure 3

Detection of transgene (TGF-β, FGF-2) expression via immunocytochemistry in hACL fibroblast cultures transduced using rAAV-coated PCL films. The PCL films were coated with rAAV-hTGF-β or rAAV-hFGF-2 or left without a vector coating before they were applied to the cultures as described in Figure 2, and the expression of (A) TGF-β and (B) FGF-2 was examined after 21 days via immunocytochemistry (magnification ×4; scale bars: 300 μm; all representative data) with an analysis of the integrated densities using ImageJ software, as described in the Materials and Methods section. Abbreviations: −/−—lack of vector and PCL film; −/NG—uncoated, ungrafted PCL films; −/G—uncoated, pNaSS-grafted PCL films; TGF-β/−—film-free rAAV-hTGF-β; TGF-β/NG—rAAV-hTGF-β-coated, ungrafted PCL films; TGF-β/G—rAAV-hTGF-β-coated, pNaSS-grafted PCL films; FGF-2/−—film-free rAAV-hFGF-2; FGF-2/NG—rAAV-hFGF-2-coated, ungrafted PCL films; FGF-2/G—rAAV-hFGF-2-coated, pNaSS-grafted PCL films. Statistically significant versus the −/− condition (*** p ≤ 0.001) (dots represent single replicates).