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. 2023 Jul 6;24(13):11140. doi: 10.3390/ijms241311140

Figure 5.

Figure 5

Evaluation of the gene expression profiles via real-time RT-PCR of the matrix components in hACL fibroblast cultures transduced using rAAV-coated PCL films. The PCL films were coated with rAAV-hTGF-β or rAAV-hFGF-2 or left without a vector coating before they were applied to the cultures as described in Figure 2, Figure 3 and Figure 4, and the gene expression profiles for type-I collagen (COL1A1), type-III collagen (COL3A1), decorin, and tenascin-C were measured after 21 days via real-time RT-PCR, with GAPDH serving as a housekeeping gene, as described in the Materials and Methods section. Ct values were obtained for each target gene and for GAPDH as a control for normalization, and fold inductions (relative to the −/− condition) were measured using the 2−ΔΔCt method. Abbreviations: −/−—lack of vector and PCL film; −/NG—uncoated, ungrafted PCL films; −/G—uncoated, pNaSS-grafted PCL films; TGF-β/−—film-free rAAV-hTGF-β; TGF-β/NG—rAAV-hTGF-β-coated, ungrafted PCL films; TGF-β/G—rAAV-hTGF-β-coated, pNaSS-grafted PCL films; FGF-2/−—film-free rAAV-hFGF-2; FGF-2/NG—rAAV-hFGF-2-coated, ungrafted PCL films; FGF-2/G—rAAV-hFGF-2-coated, pNaSS-grafted PCL films. Statistically significant versus the −/− condition (* p ≤  0.05, ** p ≤ 0.01) (dots represent single replicates).