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. 2023 Jun 30;19(6):e1011484. doi: 10.1371/journal.ppat.1011484

Fig 7. RNA is located at one end of influenza filaments.

Fig 7

A&B) Diffraction limited images of filamentous A/Udorn/72 stained with an anti-HA antibody (green) overlaid with super-resolution localisations from an array of FISH probes against the NA gene segment (red). Scale bars 1 μm. White boxes denote Archetti bodies. C) Top: Raw intensity traces (grey) of the HA signal from 68 filaments, with the average intensity profile shown as a green line. Bottom: Normalised average HA intensity from 68 filaments. D) Same as C) but for the red super-resolution signal corresponding to NA RNA from the 68 filaments. E) Top: Raw intensity traces (grey) of the RNA signal from 14 filaments with a visible Archetti body, with the average intensity profile shown as a green line. Bottom: Normalised average RNA intensity from the 14 filaments. F) Same as E) but for the RNA signal from 54 filaments with no visible Archetti body.