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. 2023 Jul 13;18(7):e0286221. doi: 10.1371/journal.pone.0286221

Fig 2. Context fear training across Experiments.

Fig 2

Mice assigned to 0.4% NaCl represented by blue symbols, mice assigned to 4.0% NaCl represented by red symbols; mice to be tested in Training Context represented by squares and solid lines, mice to be tested in Neutral Context represented by circles and dotted lines. All mice were trained in the Training Context with five, pseudorandomized mild (1 s, 0.8 mA) foot shocks during a 6 min training session. Percent freezing during training of mice in A) Experiment 1, B) Experiment 2 (grey shading), and C) Experiment 3. Baseline percent freezing averaged across the first two minutes of the training session and plotted along x-axis as 0 time point. Average percent freezing for each 30 sec period following each of the five mild foot shocks are plotted along x-axis as Post-shock Periods 1–5. Experiment 1: 0.4% NaCl females Training Context, n = 7; 0.4% NaCl females Neutral Context, n = 9; 4.0% NaCl females Training Context, n = 8; 4.0% NaCl females Neutral Context, n = 9; 0.4% NaCl males Training Context, n = 8; 0.4% NaCl males Neutral Context, n = 9; 4.0% NaCl males Training Context, n = 7; 4.0% NaCl males Neutral Context, n = 8. Experiment 2: 0.4% NaCl females Training Context, n = 9; 0.4% NaCl females Neutral Context, n = 8; 4.0% NaCl females Training Context, n = 8; 4.0% NaCl females Neutral Context, n = 8; 0.4% NaCl males Training Context, n = 9; 0.4% NaCl males Neutral Context, n = 8; 4.0% NaCl males Training Context, n = 8; 4.0% NaCl males Neutral Context, n = 10. Experiment 3: 0.4% NaCl females Training Context, n = 8; 0.4% NaCl females Neutral Context, n = 9; 4.0% NaCl females Training Context, n = 9; 4.0% NaCl females Neutral Context, n = 8; 0.4% NaCl males Training Context, n = 7; 0.4% NaCl males Neutral Context, n = 8; 4.0% NaCl males Training Context, n = 8; 4.0% NaCl males Neutral Context, n = 8. Data are graphed as mean ± 95% confidence interval. ✣indicates p<0.05 difference between males to be tested in Neutral Context and consuming 0.4% and 4.0% NaCl diets at indicated time points.