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. 2023 Jun 22;15(13):2839. doi: 10.3390/nu15132839

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Impact of 3T3-L1 adipocyte-conditioned medium (CM) on MCF-7 cell phenotype. (a) Number of live cells in MCF-7 cells treated for 48 h with CM. Controls (C) were treated with DMEM 3% CS-FBS (basal conditions). Trypan blue negative cells were counted through a Countess^® II Automated Cell Counter at day 0 and after 48 h of treatment. (b) MTT assay performed 48 h after CM treatment. (c) Soft agar growth assays in MCF-7 cells treated or not with CM. After 14 days of growth, colonies ≥ 50 μm were counted. Right panel, representative pictures. (d) Transmigration and (e) invasion assays in MCF-7 cells treated as indicated. Right panel, representative pictures. * p < 0.05; *** p < 0.001.