Figure 5.

Effects of 3T3-L1 adipocyte-derived conditioned medium (CM) on CEBP-β phosphorylation and expression in BC cells. Immunoblotting showing (a) total protein CEBP-β and (b) phosphorylated (p) CEBP-β levels in MCF-7 BC cells after 48 and 72 h exposure to CM. Controls (−) were treated with DMEM 3% CS-FBS (basal conditions). Immunoblotting showing (c) total protein CEBP-β and (d) phosphorylated (p) CEBP-β levels in MDA-MB-231 BC cells treated as indicated. GAPDH was used as a control for equal loading and transfer. Italicized numbers below the blots represent the mean of the band optical density expressed as fold over CM-untreated cells.