Table 4.
S. No | Antioxidant Assay Method | Plant Part | Type of Extract | Findings | Reference |
---|---|---|---|---|---|
1. | DPPH assay | Whole-plant powder | Methanolic (MT) and aqueous | At 300 µg/mL Aqueous extract inhibited 96%; MT inhibited 73% |
[117] |
Seed | Chloroform (CF), ethyl acetate (EA), acetone (AT) and MT extracts | At 200 µg/mL MT inhibited 87.83%; AT—84.55% EA—86.41% CF—80.44% |
[152] | ||
Aerial parts | Hexane (HE), EA, CF extracts | IC50 (µg/mL): Hexane—>1000, EA—140, CF—435 | [154] | ||
Seed | MT extracts | At 0.1 mg/mL, MT inhibited 74% | [155] | ||
2. | Superoxide anion radical scavenging activity
|
Whole-plant powder | MT and aqueous | At 300 µg/mL, aqueous extract inhibited 74% while MT inhibited 85% | [117] |
Seed | Petroleum Ether (PE), CF, EA, AT, and MT extracts | At 200 µg/mL, MT inhibited 66.79%, AT—60.35%, EA—68.29%, CF—64.04% | [152] | ||
3. | Hydroxyl scavenging activity- deoxyribose assay | Whole-plant powder | MT and aqueous | At 300 µg/mL, Aqueous extract inhibited 83% while MT inhibited 94% | [117] |
4. | Modified thiobarbituric acid reactive species assay | Whole-plant powder | MT and aqueous | At 300 µg/mL, Aqueous extract inhibited 86% while MT inhibited 78% | [117] |
5. | H2O2 scavenging assay | Seed | PE, CF, EA, AT, and MT extracts | At 200 µg/mL, MT inhibited 87.67%, AT—75.85%, EA—78.86%, CF—85.57% | [152] |
6. | ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay | Seed | PE, CF, EA, AT, and MT extracts | At 200 µg/mL, MT inhibited 85.70%, AT—84.77%, EA—87.17%, CF—88.22% | [152] |
7. | β-Carotene bleaching test | Aerial parts | Hexane, EA, CF extracts | IC50 (µg/mL) at 60 min: Hexane—>100, EA—38.03, CF—>100 | [154] |