Table 1.
Bacteria and plasmids used in this study.
| Strain and plasmid | Description | Reference |
|---|---|---|
| PATH554 | The wild-type strain of P. thiaminolyticus PATH554 | This study |
| DH5α | E. coli DH5α as a host for cloning of the ant(6)-If gene | Our laboratory collection |
| BL21 | E. coli BL21 as a host for expression of the ant(6)-If gene | Our laboratory collection |
| ATCC 29213 | S. aureus ATCC 29213 as the quality control for antimicrobial susceptibility testing | Our laboratory collection |
| pMD19-ant(6)-If/DH5α | DH5α carrying the recombinant plasmid pMD19-ant(6)-If | This study |
| pCold I-ant(6)-If/BL21 | BL21 carrying the recombinant plasmid pCold I-ant(6)-If | This study |
| pMD19 | Cloning vector for the ant(6)-If gene with its upstream promoter region, AMPr | Our laboratory collection |
| pCold I | Expression vector for the ORF of the ant(6)-If gene, AMPr | Our laboratory collection |
AMPr: ampicillin resistance.