Table 3.
Primers used to clone the ant(6)-If gene.
| Primera | Sequence (5′ → 3′)b | Restriction endonuclease | Vector | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|---|
| pro-ant(6)-If-F | GGGCAATGGTTTCGTTACTGAGAAG | pMD19 | 60 | 1,096 | |
| pro-ant(6)-If-R | GCGCAAAAATATAACCGCCCACTAA | pMD19 | 1,096 | ||
| orf-ant(6)-If-F | CGGGATCCCTGGTGCCGCGCGGCAGCTTGAGAAATGAACAAGAAATGATGAAC | BamHI + Thrombin | pCold I | 60 | 889 |
| orf-ant(6)-If-R | CCAAGCTTTCACTTCCATGAATTTCGAATATG | HindIII | pCold I | 889 |
a
Primers started with “pro” were used to clone the ant(6)-If gene with its promoter region; primers started with “orf” were used to clone the ORF of the ant(6)-If gene.
b
The underlined sequences indicated the restriction endonuclease sites.