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. 2023 Jun 28;28(13):5076. doi: 10.3390/molecules28135076

Figure 6.

Figure 6

Effects of praeruptorin A and B on the mRNA expression of proinflammatory genes. Each compound and IL-1β were added to the medium of rat hepatocytes and further incubated for 4 h. Then, total RNA was extracted and subjected to RT–qPCR. The levels of each mRNA were measured in triplicate and normalized to the EF-1α mRNA level: (A) iNOS mRNA; (B) Tnf mRNA; (C) Ccl20 mRNA; and (D) Il1r1 mRNA. Relative mRNA levels are shown as the mean ± SD (n = 3) of the resulting percentage. In the presence of IL-1β alone, the mRNA level measured was set as 100%. When IL-1β was not added or when each compound alone was added to the medium, amplification was not detected. * p < 0.05 and ** p < 0.01 versus IL-1β alone.