Table 3.
Antibodies and staining used to assess protein expression in cells.
| Target | Primary Antibody | Dilution | Secondary Antibody | Dilution |
|---|---|---|---|---|
| Aggrecan | mouse-anti-human, R&D systems, Minneapolis, MN, USA |
1:30 | donkey-anti-mouse; Cy3, Invitrogen, Carlsbad, CA, USA | 1:200 |
| Collagen type 1 | goat-anti-human, (COL1A1 chain), Abcam, Cambridge, UK | 1:50 | donkey-anti-goat; Cy3, Invitrogen, Carlsbad, CA, USA | 1:200 |
| Collagen type 2 | rabbit-anti-human, Acris Laboratories, Hiddenhausen, Germany |
1:50 | donkey-anti-rabbit, Alexa Fluor 488, Invitrogen, Carlsbad, CA, USA | 1:200 |
| Decorin | rabbit-anti-human, OriGene Rockville, MD, USA | 1:50 | donkey-anti-rabbit; Alexa-Fluor488, Invitrogen, Carlsbad, CA, USA | 1:200 |
| IL-10 | rabbit-anti-human, PeproTech, Cranbury, NJ, USA | 1:30 | donkey-anti-rabbit, Alexa Fluor 488, Invitrogen, Carlsbad, CA, USA | 1:200 |
| IL-24 | rabbit-anti-human, Invitrogen, Carlsbad, CA, USA | 1:30 | donkey-anti-rabbit; Alexa-Fluor488, Invitrogen, Carlsbad, CA, USA | 1:200 |
| IL-24 | mouse-anti-human, R&D systems, Minneapolis, MN, USA |
1:30 | donkey-anti-mouse; Cy3, Invitrogen, Carlsbad, CA, USA | 1:200 |
| Phalloidin Alexa488 | Santa Cruz Biotechnologies, Santa Cruz, CA, USA | 1:100 | none | |
| Proteoglycans | mouse-anti-human, Chemicon International, Temecula, CA, USA | 1:70 | donkey-anti-mouse; Cy3, Invitrogen, Carlsbad, CA, USA | 1:200 |
| SOX9 | rabbit-anti-human, Merck, Darmstadt, Germany | 1:100 | donkey-anti-rabbit, Alexa Fluor 488, Invitrogen, Carlsbad, CA, USA | 1:200 |
| αsmooth muscle actin | mouse-anti-human, Sigma-Aldrich (A5228), Munich, Germany | 1:50 | donkey-anti-mouse; Cy3, Invitrogen, Carlsbad, CA, USA | 1:200 |
Cy3: cyanine 3, COL1A1: collagen type 1 alpha1 chain, IL: interleukin, SOX9: SRY-box transcription factor 9.