Table 4.
Rat-specific primers used for RTD PCR in this study.
| Gene Symbol | Gene Name | Assay ID | Amplicon Length (bp) |
Efficiency | NCBI Gene Reference |
|---|---|---|---|---|---|
| Acan * | Aggrecan | Rn00573424_m1 | 74 | 2.04 | NM_022190.1 |
| Cd55 | Decay accelerating factor | Rn00709472_m1 | 92 | 1.94 | NM_022269.2 |
| Cd59 | Protectin/MAC inhibitory protein | Rn00563929_m1 | 80 | 2.05 | NM_012925.1 |
| Col1a1 | Collagen type 1, alpha 1 | Rn01463848_m1 | 115 | 2.01 | NM_053304.1 |
| COL2A1 ** | Collagen type 2, alpha 1 | Ec03467411_m1 | 81 | 2.17 | NM_001081764.1 |
| Hmox1 | Heme oxygenase-1 | Rn00561387_m1 | 132 | 2.25 | NM_012580.2 |
| Hprt1 | Hypoxanthine-Guanine-phosphoribosyltransferase | Rn01527840_m1 | 64 | 1.98 | NM_012583.2 |
| Socs3 | Suppressor of Cytokine Signaling 3 | Rn00585674_s1 | 73 | 1.90 | NM_053565.1 |
* All primers were obtained from ThermoFisher Scientific (Germany), bp: base pairs. ** this equine primer displayed high efficacy for rat samples and hence, could be used.