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. 2023 Jun 29;14:1089956. doi: 10.3389/fgene.2023.1089956

FIGURE 1.

FIGURE 1

Depth of coverage of mtDNA long-read sequencing by the Ligation kit (A). Depth of coverage and visualization by IGV of mtDNA for two control samples (ctrl1 and ctrl2) processed with the Ligation sequencing kit. Coverage profile for ctrl1 sequenced by short-reads NGS is reported for comparison. Data were obtained by processing single-amplicon PCR of the whole mtDNA according to Nextera XT protocol (Illumina), and sequencing mtDNA libraries on MiSeq platform (read length 2 × 150 bp, paired-end), as previously described (Legati et al., 2021). (B). Depth of coverage and visualization by IGV of mtDNA for samples with single large deletions (del1, del2, del3, del4, del5, del6, del7 del8, and del9) processed with the Ligation sequencing kit.