FIGURE 3.

Depth of coverage and alignment visualization of mtDNA long-read sequencing by the Rapid kit (A). Depth of coverage for a control (ctrl1) and two samples with single deletion (del1 and del2) sequenced using the Rapid Sequencing Kit. The box reports a detail of the mtDNA single large deletion in sample del1, easily identifiable by a drop in the coverage. (B). Depth of coverage of mtDNA long-read sequencing for samples with multiple deletions (multidel1, multidel2, multidel3, and multidel4). Reads containing different deletions in the sample multidel1 are clearly visible (black arrows).