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. 2023 Jun 29;14:1089956. doi: 10.3389/fgene.2023.1089956

TABLE 2.

Comparison between the ONT ligation and ONT rapid sequencing kits.

	Ligation Kit	Rapid Kit
Average N° of reads per sample ^a	240,117	176,790
Average N° of reads mapped on chrM ^a	2,702	2,184
Average mtDNA read length ^a	6,220 bp	4,403 bp
Average depth of mtDNA coverage ^a	921x	545x
mtDNA linearization	Endonuclease (specific site)	Transposase (random)
mtDNA single large deletion	OK	OK
mtDNA single large deletion	Warning: endonuclease’s cutting site must fall outside the single large deletion	OK
mtDNA multiple deletions	OK	OK
mtDNA multiple deletions	Warning: endonuclease’s cutting site can fall within multiple deleted regions introducing bias in their detection	OK
mtDNA complex rearrangements	NO	OK
mtDNA complex rearrangements	The linearization of mtDNA by endonuclease in a specific site can alter molecules containing complex alterations	OK

^{^a}

Average values were calculated from NGS, data of samples ctrl1, del1, del2 (R9.4.1 flowcell, no multiplexing).