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. Author manuscript; available in PMC: 2023 Jul 13.
Published in final edited form as: Biomed Mater. 2021 May 28;16(4):10.1088/1748-605X/ac01b0. doi: 10.1088/1748-605X/ac01b0

Figure 6. Cytokine secretion from THP-1 cells and M0 macrophages cultured in 2D, soft, and stiff PGANB hydrogels.

Figure 6.

(A) Immunostaining for polarization markers expressed in THP-1 cells and M0 macrophages at day 7. (B Heat-map of M1 and associated cytokines measured by human cytokine array C3 kit using conditioned media collected on day 4 of culture. Results were shown as adjusted mean volume (OD x mm2) ± SD of two repeats on the antibody membranes. The average intensity of the pixels in background volume was calculated and subtracted from each pixel in all standard and samples using ImageJ. (C) ELISA measurement of secreted IL-8.