Extended Data Fig. 1. Binding properties for Trp2 specific clones and purification.

a Serial diluted Trp2/H2-K^b monomer staining of scFv2, scFv12, scFv13 and scFv19 individually displayed on yeasts. b Trp2/H2-K^b specific clones scFv2, scFv12, scFv13 and scFv19 were expressed as scFv format from expi293 cells and purified by SEC and resolved as monodisperse peak. The MW of the scFv is 27 kDa. The purified proteins exhibited as single band by SDS–PAGE. c The IgG format of Trp2/H2-K^b specific clones are expressed from expi293 cells and purified from protein G column. The purified proteins exhibited heavy chain and light chain on SDS–PAGE gel under reducing condition. d Serial diluted mIgG2a 13 and 25-D1.16 IgG2a incubated with EL4 cells pulsed with 10 µM Trp2 peptide. e Serial diluted mIgG2a 13 and 25-D1.16 IgG2a incubated with EL4 cells pulsed with 10 µM OVA peptide.

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