Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 29;25(7):989–1003. doi: 10.1038/s41556-023-01163-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 9 — (a, b) Representative IB for indicated proteins in NIH3T3 cells silenced for (a) Dnml1 or (b) Mff and expressing mCherry-tagged NDRG1^WT. Blots are representative of n = 3 independent experiments obtaining similar results. (c) IB for FLAG and mCherry in OA-treated (2.5 h) NIH3T3 cells co-expressing FLAG-NDRG1^WT and mCherry-CDC42^WT or mCherry-CDC42^Thr17Asn mutant and subjected to pulldown of FLAG using FLAG M2 agarose (n = 4 independent experiments). Quantification for relative enrichment of mCherry in FLAG pulldowns was calculated by normalizing the densitometry value of mCherry to the densitometry value of FLAG. (d) Representative IB for indicated proteins in NIH3T3 cells silenced for Cdc42 and expressing mCherry-tagged NDRG1^WT. CDC42 blot is representative of n = 3 independent experiments obtaining similar results. (e) Representative live-cell imaging in cells expressing mcherry-CDC42^WT or dominant negative mcherry-CDC42^Thr17Asn mutant in presence of MitoTracker green to label mitochondria. Red arrowheads: CDC42 (mCherry). White arrowheads: CDC42 (mCherry)/mitochondria (MitoTracker) contacts prior to fission. Yellow arrowheads: divided mitochondria after contact with CDC42 (mCherry). Magnified insets are shown. Please refer to Supplementary Video 14 (mCherry-CDC42^WT/MitoTracker), and Supplementary Video 15 (mCherry-CDC42^Thr17Asn/MitoTracker). (f) Graphical representation for duration of interaction between mCherry-CDC42^WT or mCherry-CDC42^Thr17Asn and mitochondria (MitoTracker), and whether interactions lead to division or are futile. (g) Quantification for mean duration of interaction (mCherry-CDC42^WT 10 cells, and mCherry-CDC42^Thr17Asn 7 cells from n = 3 independent experiments. Each tracked cell was monitored on an independent plate). Ponceau is loading control. Individual replicates and means are shown. **P < 0.01, two-tailed unpaired Student’s t-test (c and g). ns=not significant. Please refer to Supplementary Table 10_statistical summary. Source numerical data are available in SourceData_Table 1, and unprocessed blots are available in Source Data Extended Data Fig. 9.

Source data