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. 2023 Jun 10;26(7):107095. doi: 10.1016/j.isci.2023.107095

Figure 3.

Figure 3

IHC-based correlation analyses of CD8+ T cells vs. immune and viral defense markers in LUAD TMAs

(A) LUAD H-score-based Spearman’s rank correlation similarity matrix, one minus Spearman’s rank correlation-based clustering (n = 128).

(B) LUAD H-score-based correlation forest plot for panel markers vs. CD8α H-score, Spearman’s rank correlation (rho with 95% confidence interval depicted). ∗ Indicates an adjusted p value less than 0.05 after FDR 0.05 multiple comparisons correction two-stage linear step-up procedure of Benjamini, Kriegerm, and Yekutieli (n = 128).

(C) LUAD H-score-based correlation forest plot for panel markers vs. CD8α infiltration score, Spearman’s rank correlation (rho with 95% confidence interval depicted). ∗ Indicates an adjusted p value less than 0.05 after FDR 0.05 multiple comparisons correction two-stage linear step-up procedure of Benjamini, Kriegerm, and Yekutieli (n = 128).

(D) Representative NSCLC LUAD, paraffin archive to show the prioritized 4-marker IHC panel with H&E for morphologic reference. All IHC stains were scored by modified H-score with a primary focus on CD8 T cells and tumor infiltrative pattern. Tumors are arranged top to bottom by high to low CD8α H-scores, respectively. 100x–400x total magnification. Representative scale bar provided in top left image of figure panel.