Table 4.

The anti-elastase activity of C. latifolia extract.

Extract	Percentage of inhibition (µg/mL) in a concentration of:							IC50 (µg/mL)
	2.34	4.69	9.375	18.75	37.5	75	150
REE	31.76 ± 4.22	36.91 ± 0.59	45.14 ± 0.27	49.09 ± 0.73	52.87 ± 3.29	57.90 ± 2.04	–	23.84*
SEE	36.14 ± 1.78	40.88 ± 1.05	44.12 ± 6.41	51.44 ± 1.62	56.21 ± 1.84	60.75 ± 3.77	–	16.89*g
LEE	35.29 ± 0.78	37.91 ± 1.49	40.21 ± 3.25	44.35 ± 3.06	48.45 ± 4.79	51.32 ± 2.69	–	58.31*e
REAE	26.12 ± 5.95	31.38 ± 4.06	39.85 ± 1.41	45.72 ± 1.53	52.04 ± 4.78	61.12 ± 1.59	66.06 ± 1.01	27.91*
SEAE	24.66 ± 4.31	27.87 ± 3.28	33.10 ± 2.34	41.34 ± 3.00	45.84 ± 2.22	51.35 ± 0.86	59.12 ± 2.47	58.11*c
LEAE	13.82 ± 4.21	17.49 ± 2.65	21.13 ± 2.93	26.95 ± 3.77	30.26 ± 3.27	36.13 ± 4.68	45.37 ± 2.03	>150*
RHE	31.37 ± 6.86	36.72 ± 3.33	46.93 ± 1.08	48.96 ± 1.79	54.39 ± 1.10	61.27 ± 0.59	68.79 ± 1.68	19.22*b
SHE	14.91 ± 4.41	19.07 ± 3.72	23.91 ± 0.19	33.54 ± 3.63	40.36 ± 1.08	48.14 ± 1.69	52.60 ± 8.00	106.35*
LHE	3.69 ± 1.91	7.70 ± 6.81	12.8 ± 7.19	17.4 ± 5.79	23.2 ± 1.64	27.1 ± 2.97	30.0 ± 1.35	>150*
Quercetin	IC50 (4.88 µg/mL)

Note: Concentration series on positive control quercetin (1.5 to 13.5 µg/mL). The percentage inhibition (%) of elastase at each concentration is expressed as mean ± SD (n = 3). *indicates the data is extensive different with p ≤ 0.05 level (Tukey HSD). ^abc..Alphabetical differences indicated that the data were not extensive different with a level of p ≤ 0.05 (Tukey HSD).