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. 2023 Jul 13;14:4084. doi: 10.1038/s41467-023-39404-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Study protocol of NASH model with choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) and the induction of lipophagy with AAV-mediated gene delivery into hepatocytes. b Electron micrographs of lipophagy-induced liver tissues. Scale bars, 1 µm. c–e Food intake (c), body weight (d) and liver/body weight (LW/BW) ratios (e) of lipophagy-induced NASH model mice. f Hematoxylin/eosin or oil red O (ORO) staining of liver sections. Scale bars, 1 mm. g Liver TG and NEFA content. h Serum cholesterol and triglyceride (TG) in 4 major fractions and NEFA contents. i Serum AST and ALT levels. j, k RNA sequence and gene set enrichment analysis of liver tissues. l Immunoblots assessing the expression of αSMA in liver tissues. m, n Immunohistochemistry of αSMA (m) and sirius red staining (n) of liver sections and morphometry analysis of signal positive area. Scale bars, 200 µm. All data are presented as mean ± SD (n = 6 per group). P values calculated by two-sided unpaired t-test. Source data are provided as a Source data file.