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. 2023 Jul 13;14:4084. doi: 10.1038/s41467-023-39404-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Flow cytometry for Lamp1 in the surface of control and lipophagy-induced HepG2 cells. SYT7 or TRPML1 was knockout by CRISPR-Cas9 (n = 3 per group). b Flow cytometry to evaluate whole cell expression level of LAMP1 in saponin-treated cells. c TG in the medium of control and lipophagy-induced HepG2 cells. Cells were cultured with FFAs (OA + PA) containing medium for 12 h and then maintained in FBS-free medium for 6 h (n = 9 per NTC group, n = 6 per SYT7 and TRPML1 group). d Lysosome acid lipase activity in control and lipophagy-induced HepG2 cells (n = 3 per group). e Lamp1, LysoTracker Red DND-99 and BODIPY co-staining in control and lipophagy-induced HepG2 cells. Scale bar, 10 mm. f Flow cytometry for LysoSensor Green DND-189 staining of control and lipophagy-induced HepG2 cells (n = 3 per group). All data are presented as mean ± SD. P values calculated by two-sided unpaired t-test. Source data are provided as a Source data file.