Table 1.

Examples of top-ranked computationally predicted patient mutations confirmed to disrupt complex formation.

Protein	Interaction partner	Mutation	ΔK* (Log10) scorea	Reference
p53	DNA consensus sequence	R248Q	−4.46b	Merabet et al.2
		R248W	−4.54b	Merabet et al.
		R249S	−4.60b	Merabet et al.
		R273C	−7.86c	Garg et al.5
		R273H	−7.74c	Garg et al.
		R273L	−7.55c	Garg et al.
	ASPP2/53BP2	R248W	−53.49	Gorina et al.23
		R249S	−4.14	Gorina et al.
		R273H	−5.01	Gorina et al.
ERK2	DUSP6	D321N	−8.76	Brenan et al.3 Taylor et al.36
p16	CDK6	G23D	Xd	McKenzie et al.37
		M53I	X	Harland et al.38
		D84G	−5.19	Yarbrough et al.39
		D84H	X	Ruas et al.40
		D84N	−5.26	Ruas et al.
		D84V	X	Yarbrough et al.
		D84Y	X	Ruas et al.
		R87P	X	Yarbrough et al.
smad4	smad2	R361C	−4.68	Shi et al.4
		D537E	−7.03	Shi et al.
		D537Y	X	Gori et al.41

^aDifference of mutant Log₁₀ K* score in comparison to wildtype score.

^bChain A results.

^cChain B results.

^dBinding was completely disrupted for the mutant and thus no difference to the wildtype score could be calculated.