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. 2023 Jul 13;13:259. doi: 10.1038/s41398-023-02561-9

Fig. 1. Behavioral effects of Mbd2 deficiency.

Fig. 1

A Locomotion in the Open-field box was assessed for 5 min. n = 14 wild-type, n = 15 mMbd2−/−. B Self-grooming during open field. (Two-way ANOVA, main effect of genotype F(1,28) = 5.2, p = 0.0304). C Number of self-grooming bouts. (Two-way ANOVA, main effect of genotype F(1,28) = 9.62, p = 0.0044). D Average self-grooming bout duration. E Y-maze spontaneous alteration was not affected in Mbd2−/− mice (right). SAP-Spontaneous Alteration Performance, AAR-Alternate Arm Return, SAR-Same Arm Return. Exploration is expressed as number of arm entries (right). n = 17 wild-type, n = 17 Mbd2−/−. F Exploration time during object-location memory training (left) and discrimination ratio in object-location memory test (right). Discrimination ratio was significantly lower in Mbd2−/− mice (Two-way ANOVA, main effect of genotype F(1,24) = 5.05, p = 0.0341; n = 13 wild-type, n = 15 Mbd2−/−). G Social interaction. Mice were introduced to a novel mouse for 5 min and interaction time was recorded. (Two-way ANOVA, main effect of genotype F(1,29) = 5.02, p = 0.0329; n = 17 wild-type, n = 16 Mbd2−/−). H Time spent in the light compartment of the Dark-Light Box (left) (Two-way ANOVA, main effect of genotype F(1,29) = 5.3, p = 0.0287; n = 17 wild-type, n = 16 Mbd2−/−) and number of entries to the light side (right). Data are presented as mean ±SEM *p < 0.05, **p < 0.01 for wild-type vs Mbd2−/−. ### p < 0.005 for wild-type over chance (50%) exploration (one-sample t-test). Since no main effect for sex was observed in any of the tests (for full data see Figs. S1–S3) behavioral data from males and females were collapsed within genotype for clarity.