Fig. 4. The PIP2-binding site promotes compact IDR conformations.
a Constructs used in SEC-SAXS experiments. b Real-space pair-distance distribution functions, or p(r) profiles, in arbitrary units (arb. units), calculated for IDR and IDRAAWAA (gray curves) as well as NTD and NTDAAWAA (blue curves). p(r) functions were scaled to an area under the curve of 1. The real-space distance distribution of IDRAAWAA yields a radius of gyration of Rg = 3.5 nm with a maximal particle dimension of Dmax = 14.5 nm (native IDR: Rg = 3.4 nm, Dmax = 14.0 nm). NTDAAWAA has a Rg = 4.5 nm and a Dmax = 19.5 nm (native NTD: Rg = 4.1 nm, Dmax = 19.0 nm). c Fit between EOM-refined IDR and NTD models and experimental scattering data, in arbitrary units (arb. units). The statistical analyses of the fits were carried out using the reduced χ2 method93 (one-tailed distribution) and CorMap64 (one-tail Schilling distribution) test methods. The determined χ2 and CorMap p values are indicated in the corresponding graphs. d Comparison between Rg values of IDR and NTD variants between random pool structure library (solid area) and EOM refined models (dotted line). Source data are provided as a Source Data file.