Skip to main content
. 2023 Jun 10;299(7):104909. doi: 10.1016/j.jbc.2023.104909

Figure 5.

Figure 5

Mitochondrial folate metabolism induces ALA exhaustion in activated HSCs.A, D, and G, differential metabolites abundance in LX-2 cells transfected with siNC, siSHMT2, or siSLC25A32 24 h after medium or TGF-β1 (5 ng/ml) stimulation (n = 3). B, E, and H, metabolite set enrichment analysis in LX2 cells transfected with siNC, siSHMT2, or siSLC25A32 24 h after medium or TGF-β1 (5 ng/ml) stimulation (n = 3). C, F, and I, heatmap of metabolites in LX-2 cells transfected with siNC, siSHMT2, or siSLC25A32 24 h after medium or TGF-β1 (5 ng/ml) stimulation (n = 3). J, LC-MS analysis of intracellular alpha-linolenic acid in knockdown SHMT2 or SLC25A32 LX-2 cells after treatment with TGF-β1 (5 ng/ml) for 24 h (n = 4). The statistical significance (∗p < 0.05, ∗∗p < 0.01) was tested by student’s unpaired t test. ALA, α-linolenic acid; HSC, hepatic stellate cell; SHMT, serine hydroxymethyltransferase.