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. 2023 Jul 13;6:719. doi: 10.1038/s42003-023-05064-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Analysis of the significant circRNAs expression clustering of basal HRVECs and high-glucose HRVECs. b circRSU1 expression in HRVECs for a period (3, 6, 12, 24, 48 and 72 h). c The amount of circRUS1 and RSU1 mRNA in HRVECs after actinomycin D treatment. d After RNase R digestion, qRT-PCR assay was conducted to detect circRSU1-expression level. RSU1 mRNA level was measured as control. e circRSU1, RSU1 mRNA, GAPDH (cytoplasm control) and U6 (nuclear control) expression levels were analyzed by qRT-PCR. f The sequence obtained from Sanger sequencing matched the sequence of circRSU1 listed in circBase. g PCR analysis revealed the presence of circRSU1 through backsplicing. Using divergent primers, circRSU1 was successfully amplified in cDNA samples while showing no amplification in gDNA samples. A negative control with GAPDH was utilized. Unpaired student’s t-test, two-way ANOVA test, and Bonferroni test were used for the statistical analyses. *p < 0.05.