Skip to main content
. 2023 Jul 13;9:59. doi: 10.1038/s41523-023-00564-9

Fig. 3. The effect of TTP488 and FPS-ZM1 treatment on tumor cell growth, viability, proliferation, and cell cycle in vitro.

Fig. 3

a–d Cell viability/proliferation assays were performed using the crystal violet assay. 4175 and 4T1 cells were treated with a range of concentrations (50–1000 nM) of FPS-ZM1 or TTP488 for 24, 48, or 72 h. a 4175 cell treatment with FPS-ZM1; (b) 4T1 treatment with FPS-ZM1; (c) 4175 treatment with TTP488; (d) 4T1 treatment with TTP488. e Cell cycle analysis of 4175 cells treated in vitro with 1000 nM of FPS-ZM1 or TTP488 for 24, 48, or 72 h. Cells were stained with propidium iodide solution and analyzed for cell cycle distribution by flow cytometry. Representative column graph showing the percentage of cells in each cell cycle. Values show mean + SD from 3–5 independent experiments performed in triplicate. Statistical analysis was performed with either one-way or two-way ANOVA. *p < 0.05.