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. 2023 Jul 13;9:59. doi: 10.1038/s41523-023-00564-9

Fig. 6. The effect of TTP488 and FPS-ZM1 on tumor-intrinsic signaling in 4175/NSG tumors.

Fig. 6

a, b Tumor lysate from 4175 tumor-bearing mice was assessed for changes in signaling pathway mechanisms due to RAGE inhibition by phospho-protein arrays. 3 pooled samples (for each of DMSO and TTP488) were assessed by the Proteome Profiler Human Phospho-Kinase Array Kit. a All phospho-proteins that displayed differences compared to DMSO control by protein array are shown. Data is shown as average of mean pixel density for each pair of duplicate spots for each cytokine. b Log2-fold change in phospho-protein differences due to RAGE inhibitor TTP488 relative to DMSO control. c Western blot validation of phospho-protein array changes was performed with tumor lysate from 4175 tumor-bearing mice (4 samples per condition). Representative images are shown for each condition. Samples were analyzed for phospho and total protein for Pyk2, STAT3 and AKT, and beta-actin loading control. d RAGE signaling in TNBC drives tumor metastasis. A schematic depicting the major signaling pathways activated by RAGE in TNBC cells leading to metastasis.