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. 2023 Jul 13;14(7):418. doi: 10.1038/s41419-023-05954-2

Fig. 1. Interleukin (IL)-10 signaling blockade aggravates lipopolysaccharide (LPS)-induced acute lung injury (ALI).

Fig. 1

A Experimental scheme: anti-IL-10 (10 µg) or anti-IL-10 receptor (IL-10R, 50 µg) were administered intranasally on days 1 and 3 following LPS (50 μg) exposure, and IgG2b and IgG1 were as controls, respectively. The mice were euthanized on day 4 for analysis (n = 7–11). B Clinical signs of mice were observed (n = 7–11). C On day 4, post-LPS administration, BALF was collected from anti-IL-10 or anti-IL-10R (isotype control, IgG2b or IgG1) mice, and the protein concentration was determined (n = 7–11). D Differential cells in the bronchoalveolar lavage fluid were counted after Cytospin and Wright–Giemsa staining (n = 7–11). E The representative image of the HE-stained section is displayed (n = 4). Scale bars, 100 μm. F The inflammation score of the HE-stained sections. (n = 4). B Two-way ANOVA was used for the statistical analyses. C, D, F Student’s t-test was used for the statistical analyses.